Characterisation of the Dunaliella tertiolecta RbcS genes and their promoter activity in Chlamydomonas reinhardtii
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چکیده
منابع مشابه
Uptake of HCO3 2 and CO2 in Cells and Chloroplasts from the Microalgae Chlamydomonas reinhardtii and Dunaliella tertiolecta
Mass-spectrometric disequilibrium analysis was applied to investigate CO2 uptake and HCO3 2 transport in cells and chloroplasts of the microalgae Dunaliella tertiolecta and Chlamydomonas reinhardtii, which were grown in air enriched with 5% (v/v) CO2 (high-Ci cells) or in ambient air (low-Ci cells). Highand low-Ci cells of both species had the capacity to transport CO2 and HCO3 , with maximum r...
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15 صفحه اولIdentification of genes required for hydrogenase activity in Chlamydomonas reinhardtii.
The eukaryotic green alga, Chlamydomonas reinhardtii, produces H(2) under anaerobic conditions, in a reaction catalysed by an [FeFe]-hydrogenase. To identify genes that influence H(2) production in C. reinhardtii, a library of 6000 colonies on agar plates was screened with sensitive chemochromic H(2)-sensor films for clones defective in H(2) production. Two mutants of particular interest were f...
متن کاملIsolation of Intact Chloroplasts from Dunaliella tertiolecta.
Cells of Dunaliella tertiolecta from the log phase of growth were broken by rapid extrusion at low pressure through a Yeda press and the chloroplasts were isolated by centrifugation through a Percoll gradient. Osmolarity of the growth media, the suspending media, and the Percoll gradient was kept identical to minimize change in chloroplast volume and mitochondrial entrapment. The isolated intac...
متن کاملMutations inactivating mitochondrial genes in Chlamydomonas reinhardtii.
Chlamydomonas reinhardtii is now becoming a useful model for the study of mitochondrial genetics in a photosynthetic organism. The small (15.8 kb) mitochondrial genome C. reinhardtii has been sequenced completely and all the genes have been identified. Several mutants inactivated in mitochondrial genes encoding components of the respiratory complexes I, III and IV have been characterized at the...
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ژورنال
عنوان ژورنال: Plant Cell Reports
سال: 2004
ISSN: 0721-7714,1432-203X
DOI: 10.1007/s00299-004-0884-x